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Synthesis, characterization, in vitro degradation and cytotoxicity of polyphosphazenes containing N ‐ethoxypyrrolidone side groups
Author(s) -
Bi Yunmei,
Yin Yuefan,
Huang Rong,
Li Yanmei
Publication year - 2010
Publication title -
polymer international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 105
eISSN - 1097-0126
pISSN - 0959-8103
DOI - 10.1002/pi.2720
Subject(s) - polyphosphazene , phosphazene , hydrolysis , cytotoxicity , polymer , nuclear chemistry , alkoxy group , differential scanning calorimetry , chemistry , glass transition , degradation (telecommunications) , polymer chemistry , mtt assay , materials science , organic chemistry , in vitro , biochemistry , alkyl , telecommunications , physics , computer science , thermodynamics
A new biodegradable polyphosphazene (PYRMP) containing N ‐ethoxypyrrolidone and methoxyethoxyethoxy side groups was synthesized via a route of macromolecular substitution. The synthetic method of poly{bis[2‐(2‐oxo‐1‐pyrrolidinyl)ethoxy]phosphazene} (PYRP) was improved. The thermal properties of the polymers were investigated using differential scanning calorimetry. Degradation studies were carried out in vitro with varying pH conditions. The in vitro cytotoxicity of PYRMP and its hydrolysis products was evaluated using the methyl tetrazolium (MTT) cytotoxicity test in HepG2 cell culture. PYRMP and PYRP have low glass transition temperatures of −68.8 and −59.6 °C, respectively. The polymers show a higher degradation rate at pH = 5.0 than at both pH = 7.4 and 8.0. The degradation process of PYRMP in different buffer solutions is discussed. The MTT test reveals that PYRMP at concentrations below 800 µg mL −1 and its hydrolysis products are non‐toxic to HepG2 cells. Moreover, the hydrolysis products diluted 10 times are able to promote cell proliferation. This study shows that polyphosphazene containing N ‐ethoxypyrrolidone subsituents provides interesting perspectives for various biomedical applications. Copyright © 2009 Society of Chemical Industry