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Determination of molar mass and solution properties of cationic hydroxyethyl cellulose derivatives by multi‐angle laser light scattering with simultaneous refractive index detection
Author(s) -
Gao Wei,
Liu X Michael,
Gross Richard A
Publication year - 2009
Publication title -
polymer international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 105
eISSN - 1097-0126
pISSN - 0959-8103
DOI - 10.1002/pi.2636
Subject(s) - molar mass , polymer , cationic polymerization , chemical engineering , materials science , radius of gyration , static light scattering , chemistry , chromatography , analytical chemistry (journal) , polymer chemistry , organic chemistry , dynamic light scattering , nanotechnology , nanoparticle , engineering
BACKGROUND: A complete understanding of the molar mass and solution properties of raw materials in bio/pharmaceutical products under bio‐application and natural conditions ensures process control, product performance and quality. Biopolymers including polymeric cationic hydroxyethyl cellulose derivatives (e.g. Polyquaterium‐10 or Polymer JR) have long been used in health care formulations including shampoos, lotions, eye drops and contact lens multi‐purpose solutions. Previously reported molar masses and conformation of Polymer JR were based on size exclusion chromatography‐related techniques, which required highly concentrated buffered salt solutions and organic solvent modifiers to prevent undesirable interactions, and did not represent the isotonic conditions in products and applications. RESULTS: This paper describes the characterization of Polymer JR in saline using a new approach that combines micro‐batch mode multi‐angle laser light scattering with simultaneous refractive index measurements (MB‐MALLS‐RI). Mass‐average molar mass, z ‐average radius of gyration and second virial coefficient values in phosphate buffer saline (PBS) were obtained and are discussed in detail. CONCLUSION: The molar mass and solution properties of Polymer JR in PBS, with the same pH and ionic strength as most health care solution products, can be characterized using the MB‐MALLS‐RI technique. The approach is practical and can be widely used for the analysis of other cationic biopolymers. Copyright © 2009 Society of Chemical Industry