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Synthesis and spectroscopic characterization of superparamagnetic beads of copolymers of methacrylic acid, methyl methacrylate and ethylene glycol dimethacrylate and their application to protein separation
Author(s) -
Bayramoǧlu Gülay,
Arıca M Yakup
Publication year - 2008
Publication title -
polymer international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 105
eISSN - 1097-0126
pISSN - 0959-8103
DOI - 10.1002/pi.2313
Subject(s) - ethylene glycol dimethacrylate , differential scanning calorimetry , materials science , methacrylic acid , superparamagnetism , ethylene glycol , polymer chemistry , suspension polymerization , polymerization , methyl methacrylate , methacrylate , polymer , chemical engineering , nuclear chemistry , chemistry , magnetization , composite material , physics , quantum mechanics , magnetic field , engineering , thermodynamics
Novel magnetic beads were prepared from methacrylic acid, methyl methacrylate and ethylene glycol dimethacrylate in the presence of ferric ions via suspension polymerization. After polymerization, thermal treatment of the Fe(III)‐ion containing beads with Fe(II) ions under alkaline condition resulted encapsulation of Fe 3 O 4 crystals within the polymer matrix. The magnetic beads were characterized by surface area measurement, swelling tests, electron spin resonance (ESR), differential scanning calorimetry (DSC), differential thermal analysis (DTA) and scanning electron microscopy (SEM). To evaluate the magnetic properties of the composite beads, Mössbauer spectra measurements were carried out. ESR and Mössbauer spectroscopy data revealed that the beads were highly superparamagnetic. The DSC data of the magnetic beads also showed an enhanced glass transition temperature owing to the presence of the Fe 3 O 4 crystals within the polymer network. In addition, the presence of functional carboxyl groups on the surface permits further modifications in future applications. The magnetic beads were used for separation of human serum albumin and cytochrome c from aqueous solution in a batch system. Copyright © 2007 Society of Chemical Industry