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Preparation and characterization of hyaluronan/chitosan scaffold cross‐ linked by 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide
Author(s) -
Deng Yu,
Liu Dengru,
Du Guocheng,
Li Xiufen,
Chen Jian
Publication year - 2007
Publication title -
polymer international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 105
eISSN - 1097-0126
pISSN - 0959-8103
DOI - 10.1002/pi.2197
Subject(s) - carbodiimide , fourier transform infrared spectroscopy , chitosan , differential scanning calorimetry , polymer chemistry , nuclear chemistry , scaffold , materials science , amide , scanning electron microscope , chemical engineering , chemistry , organic chemistry , composite material , biomedical engineering , medicine , physics , engineering , thermodynamics
A novel biocompatible scaffold was prepared by cross‐linking hyaluronan (HA) and chitosan (CS). The carboxyl groups of HA were activated by 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide (EDC) and then cross‐linked with amino groups of CS by forming amide bonds. The HA/CS scaffold thus prepared was characterized using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and differential scanning calorimetry. FTIR spectra showed that the absorbance of the amide (1550 cm −1 ) and carbonyl (1633 cm −1 ) bond in the cross‐linked scaffold was stronger than that in HA or CS. SEM micrographs showed that the cross‐linked scaffold produced at low EDC concentration had an intertwisted ribbon‐like microstructure, while the product prepared at higher EDC concentration had a porous structure. The concentration of EDC in the reaction system greatly affected the structure and properties of the HA/CS scaffold. The prepared scaffold could strongly resist degradation by hyaluronidase, free radicals in vitro and stress. Copyright © 2007 Society of Chemical Industry