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Human serum albumin adsorption on poly[(glycidyl methacrylate)‐ co ‐(methyl methacrylate)] beads modified with a spacer‐arm‐attached L ‐histidine ligand
Author(s) -
Bayramoǧlu Gülay,
Şenel Ayşegül Ülkü,
Yalçın Emine,
Arica M. Yakup
Publication year - 2006
Publication title -
polymer international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 105
eISSN - 1097-0126
pISSN - 0959-8103
DOI - 10.1002/pi.1905
Subject(s) - glycidyl methacrylate , adsorption , ionic strength , polymer chemistry , human serum albumin , langmuir adsorption model , methyl methacrylate , chemistry , nuclear chemistry , desorption , polymerization , materials science , chromatography , organic chemistry , polymer , aqueous solution
Poly(GMA/MMA) beads were synthesized from glycidyl methacrylate (GMA) and methyl methacrylate (MMA) in the presence of a cross‐linker (i.e. ethyleneglycol dimethacrylate) (EGDMA) via suspension polymerization. The epoxy groups of the poly(GMA/MMA) beads were converted into amino groups with either ammonia or 1,6‐diaminohexane (i.e. spacer‐arm). An L ‐histidine ligand was then covalently immobilized on the aminated (poly(GMA/MMA)‐AH) and/or the spacer‐arm attached (poly(GMA/MMA)‐SAH) beads using glutaric dialdehyde as a coupling agent. Both affinity adsorbents were used in human serum albumin (HSA) adsorption/desorption studies under defined pH, ionic strength or temperature conditions in a batch reactor. The spacer‐arm attached affinity adsorbent resulted in an increase in the adsorption capacity to HSA when compared to the aminated counterpart (i.e. poly(GMA/MMA)‐AH). The maximum adsorption capacities of the affinity adsorbents were found to be significantly high, i.e. 43.7 and 80.2 mg g −1 (of the beads), while the affinity constants, evaluated by the Langmuir model, were 3.96 × 10 −7 and 9.53 × 10 −7 mol L −1 for poly(GMA/MMA)‐AH and poly(GMA/MMA)‐SAH, respectively. The adsorption capacities of the affinity adsorbents were decreased for HSA by increasing the ionic strength, adjusted with NaCl. The adsorption kinetics of HSA were analysed by using pseudo‐first and pseudo‐second‐order equations. The second‐order equation fitted well with the experimental data. Copyright © 2005 Society of Chemical Industry

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