Open AccessMonocarboxylate transporter expression at the onset of skeletal muscle regeneration
Author(s) -
Washington Tyrone A.,
Brown Lemuel,
Smith Dameon A.,
Davis Gina,
Baum Jamie,
Bottje Walter
Publication year - 2013
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.1002/phy2.75
Subject(s) - skeletal muscle , sarcolemma , myocyte , monocarboxylate transporter , medicine , endocrinology , lactate dehydrogenase , gene expression , dysferlin , cytochrome c oxidase , myod , myogenin , biology , myogenesis , chemistry , mitochondrion , biochemistry , transporter , gene , enzyme
The onset of skeletal muscle regeneration is characterized by proliferating myoblasts. Proliferating myoblasts have an increased energy demand and lactate exchange across the sarcolemma can be used to address this increased demand. Monocarboxylate transporters ( MCT s) are involved in lactate transport across the sarcolemma and are known to be affected by various physiological stimuli. However, MCT expression at the onset of skeletal muscle regeneration has not been determined. The purpose of this study was to determine if skeletal muscle regeneration altered MCT expression in regenerating tibialis anterior ( TA ) muscle. Male C57/ BL 6 mice were randomly assigned to either a control (uninjured) or bupivacaine (injured) group. Three days post injection, the TA was extracted for determination of protein and gene expression. A 21% decrease in muscle mass to tibia length (2.4 ± 0.1 mg/mm vs. 1.9 ± 0.2 mg/mm, P < 0.02) was observed. IGF ‐1 and MyoD gene expression increased 5.0‐fold ( P < 0.05) and 3.5‐fold ( P < 0.05), respectively, 3 days post bupivacaine injection. MCT ‐1 protein was decreased 32% ( P < 0.03); however, MCT ‐1 gene expression was not altered. There was no difference in MCT 4 protein or gene expression. Lactate dehydrogenase ( LDH )‐A protein expression increased 71% ( P < 0.0004). Protein levels of LDH ‐B and mitochondrial enzyme cytochrome C oxidase subunit decreased 3 days post bupivacaine injection. CD 147 and PKC ‐θ protein increased 64% ( P < 0.03) and 79% ( P < 0.02), respectively. MCT 1 but not MCT 4 expression is altered at the onset of skeletal muscle regeneration possibly in an attempt to regulate lactate uptake and use by skeletal muscle cells.