Open AccessAngiotensin II stimulates sympathetic neurotransmission to adipose tissue
Author(s) -
King Victoria L.,
English Victoria L.,
Bharadwaj Kalyani,
Cassis Lisa A.
Publication year - 2013
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.1002/phy2.14
Subject(s) - endocrinology , medicine , angiotensin ii , adipose tissue , brown adipose tissue , chemistry , neurotransmission , norepinephrine , saline , receptor , dopamine
Angiotensin II (AngII) facilitates sympathetic neurotransmission by regulating norepinephrine ( NE ) synthesis, release, and uptake. These effects of AngII contribute to cardiovascular control. Previous studies in our laboratory demonstrated that chronic AngII infusion decreased body weight of rats. We hypothesized that AngII facilitates sympathetic neurotransmission to adipose tissue and may thereby decrease body weight. The effect of chronic AngII infusion on the NE uptake transporter and NE turnover was examined in metabolic (interscapular brown adipose tissue, ISBAT ; epididymal fat, EF ) and cardiovascular tissues (left ventricle, LV ; kidney) of rats. To examine the uptake transporter saturation isotherms were performed using [ 3 H]nisoxetine ( NIS ). At doses that lowered body weight, AngII significantly increased ISBAT [ 3 H] NIS binding density. To quantify NE turnover, alpha‐methyl‐para‐tyrosine ( AMPT ) was injected in saline‐infused, AngII‐infused, or saline‐infused rats that were pair‐fed to food intake of AngII‐infused rats. AngII significantly increased the rate of NE decline in all tissues compared to saline. The rate of NE decline in EF was increased to a similar extent by AngII and by pair feeding. In rats administered AngII and propranolol, reductions in food and water intake and body weight were eliminated. These data support the hypothesis that AngII facilitates sympathetic neurotransmission to adipose tissue. Increased sympathetic neurotransmission to adipose tissue following AngII exposure is suggested to contribute to reductions in body weight.