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Comparative Whole Genome Sequencing of Community‐Associated Methicillin‐Resistant Staphylococcus aureus Sequence Type 8 from Primary Care Clinics in a Texas Community
Author(s) -
Lee Grace C.,
Long S. Wesley,
Musser James M.,
Beres Stephen B.,
Olsen Randall J.,
Dallas Steven D.,
Nunez Yury O.,
Frei Christopher R.
Publication year - 2015
Publication title -
pharmacotherapy: the journal of human pharmacology and drug therapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.227
H-Index - 109
eISSN - 1875-9114
pISSN - 0277-0008
DOI - 10.1002/phar.1536
Subject(s) - biology , resistome , whole genome sequencing , staphylococcus aureus , antibiotic resistance , genome , single nucleotide polymorphism , genetics , genotype , microbiology and biotechnology , gene , mobile genetic elements , antibiotics , bacteria
Study Objective Our understanding of the molecular dynamics driving the community‐associated methicillin‐resistant Staphylococcus aureus ( CA ‐ MRSA ) epidemic at the whole genome level is limited. We sought to assess the use of whole genome sequencing ( WGS ) to evaluate the genomic diversity and genotypic prediction of antimicrobial resistance of CA ‐ MRSA isolates from patients in South Texas. Design Comparative whole genome sequencing. Isolates Thirteen clinical CA ‐ MRSA isolates recovered from patients presenting with skin and soft tissue infections to nine primary care clinics in the South Texas Ambulatory Research Network between 2010 and 2013. Measurements and Main Results Comparative WGS was performed on the 13 MRSA sequence type 8 clinical isolates. We compared the resistome of genes encoding for antibiotic resistance with a phenotypically derived antibiogram using standard antimicrobial susceptibility testing. The strains differed by an average of 72 single nucleotide polymorphisms ( SNP s) per isolate in the core genome compared with FPR 3757 ( USA 300, reference strain). There were a total of 623 unique SNP s in the core genome (range 47–88 SNP s per isolate). We identified 19 nonsynonymous SNP s in genes encoding proven or putative S. aureus virulence determinants in the core genome. There was complete concordance between genotypic evidence for antimicrobial resistance and the phenotypically derived antibiogram. Conclusion Overall, although these CA ‐ MRSA isolates were similar on the level of clonal type, clinical syndrome, and geographic area, the strains were diverse at the genome level. Furthermore, our findings provide important proof‐of‐concept information for using WGS as a potential front‐end screening tool for antimicrobial resistance predictions.