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How to overcome endosomal entrapment of cell‐penetrating peptides to release the therapeutic potential of peptides?
Author(s) -
NadalBufí Ferran,
Henriques Sónia Troeira
Publication year - 2020
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.533
H-Index - 7
ISSN - 2475-8817
DOI - 10.1002/pep2.24168
Subject(s) - endosome , endocytic cycle , cytosol , cell penetrating peptide , intracellular , peptide , chemistry , endocytosis , microbiology and biotechnology , cancer cell , cell , computational biology , biochemistry , biology , cancer , enzyme , genetics
Peptides gained a renascent interest as alternative pharmaceuticals, and in particular as anticancer drugs to inhibit intracellular proteins involved in cancer pathways, inaccessible to other pharmaceutical agents. However, the use of peptides as therapeutics is still limited by difficulties in reaching the protein target located in the cytosol. Cell penetrating peptides (CPPs) are being explored as tools to deliver therapeutic peptides inside cells, but they often enter cells using endocytic pathways and become entrapped inside endosomes. Therefore, a strategy to unleash the potential of peptides as therapeutics is to control the release of CPPs from endosomes. In this review we summarize recent strategies employed to improve endosomal escape of CPPs. Lessons learned from these studies can guide the design of CPPs as tools to deliver peptide sequences able to inhibit cancer targets and accelerate their development as therapeutics.