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Equipotent enantiomers of cyclic opioid peptides at μ opioid receptor
Author(s) -
Weltrowska Grazyna,
Nguyen Thi M.D.,
Chung Nga N.,
Wilkes Brian C.,
Schiller Peter W.
Publication year - 2019
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.533
H-Index - 7
ISSN - 2475-8817
DOI - 10.1002/pep2.24078
Subject(s) - enantiomer , chemistry , stereochemistry , agonist , affinities , receptor , peptide , opioid receptor , cyclic peptide , biochemistry
Head‐to‐tail cyclized analogs of the µ opioid receptor (MOR) agonist tetrapeptides DALDA (H‐Tyr‐D‐Arg‐Phe‐Lys‐NH 2 and [Dmt 1 ]DALDA (H‐Dmt‐D‐Arg‐Phe‐Lys‐NH 2 ; Dmt = 2′,6′‐dimethyltyrosine) and their enantiomers (mirror‐image isomers) were synthesized and pharmacologically characterized in vitro. Three pairs of enantiomeric cyclic peptides with both mirror‐image isomers having equipotent MOR binding affinities but different binding affinities at the δ and κ opioid receptors were identified. The cyclic peptide enantiomers c[‐D‐Arg‐Phe‐Lys‐Tyr‐] ( 1 ) and c[‐Arg‐D‐Phe‐D‐Lys‐D‐Tyr‐] ( 2 ) showed nearly identical MOR binding affinity (1‐2 nM) and equipotent MOR antagonist activity. The results of a MOR docking study indicated a very similar binding mode of the two enantiomers with nearly complete spatial overlap of the peptide ring structures and side chain interactions with the same MOR residues. Compounds 1 and 2 represent the first pair of enantiomeric G‐protein‐coupled receptor (GPCR) ligands having multiple chiral centers, with both optical antipodes showing equal, low nanomolar receptor binding affinity.