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Separation of the collagen protein by ultrafiltration: Effects of concentration on the membrane's characteristics
Author(s) -
Scutariu RoxanaElena,
Batrinescu Gheorghe,
Nechifor Gheorghe,
Popescu Mariana,
Tenea Anda G.
Publication year - 2020
Publication title -
polymer engineering and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.503
H-Index - 111
eISSN - 1548-2634
pISSN - 0032-3888
DOI - 10.1002/pen.25486
Subject(s) - ultrafiltration (renal) , membrane , materials science , chromatography , hydrolysate , aqueous solution , viscosity , permeability (electromagnetism) , chemical engineering , chemistry , composite material , biochemistry , hydrolysis , engineering
Collagen is one of the predominant proteins found in all multicellular animals. In this paper, the performance of the ultrafiltration process (UF) for concentrating the collagen protein was studied by performing batch experiments using a membrane module with an effective area of 28 cm 2 and equipped with a regenerated cellulose membrane with a molecular weight cut‐off of 5 kDa. The feed solution consisted of aqueous solutions of bovine collagen hydrolysate with a molecular weight of 10 kDa. The effects of feed concentration (1%, 2%, 3%, 4%, and 5%) on the performance were determined. The optimum operating conditions were: a transmembrane pressure of 5 bar and a temperature of 25°C. The results showed that the permeability decreases with the increase of the volumetric concentration factor (VCF) for all five concentrated solutions of collagen tested. Also, the rejection of proteins increases with the increase in the concentration of the collagen solutions. The study confirms that the clogging phenomenon was dependent on the increase of concentration. Further, a mathematical modeling of fouling mechanism was analyzed based on Hermia's model. The concentration of the collagen solutions was also highlighted by viscosity measurements.