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Preparation and characterization of polyhydroxybutyrate‐ co ‐hydroxyvalerate/silk fibroin nanofibrous scaffolds for skin tissue engineering
Author(s) -
Lei Caihong,
Zhu Hailin,
Li Jingjing,
Li Jiuming,
Feng Xinxing,
Chen Jianyong
Publication year - 2015
Publication title -
polymer engineering and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.503
H-Index - 111
eISSN - 1548-2634
pISSN - 0032-3888
DOI - 10.1002/pen.23958
Subject(s) - fibroin , electrospinning , scaffold , materials science , fourier transform infrared spectroscopy , tissue engineering , silk , chemical engineering , nanofiber , scanning electron microscope , adhesion , composite material , polyhydroxybutyrate , polymer chemistry , biomedical engineering , polymer , medicine , engineering , biology , bacteria , genetics
Nanofibrous scaffolds were obtained by co‐electrospinning poly (3‐hydroxybuty‐rate‐ co ‐3‐hydroxyvalerate) (PHBV) and fibroin regenerated from silk in different proportions using 1,1,1,3,3,3‐hexafluoro‐2‐isopropanol (HFIP) as solvent. Field emission scanning electron microscope (FESEM) investigation showed that the fiber diameters of the nanofibrous scaffolds ranged from 190 to 460 nm. X‐ray diffraction (XRD) and Fourier transform infrared spectroscopy analysis (FT‐IR) showed that the main structure of silk fibroin (SF) in the nanofibrous scaffold was β‐sheet. Compared to the PHBV nanofibrous scaffold, the surface hydrophilicity and water‐uptake capability of the PHBV/SF nanofibrous scaffold with 50/50 were improved. The results of cell adhesion experiment showed that the fibroblasts adhered more to the PHBV/SF nanofibrous scaffold with 50/50 than the pure PHBV nanofibrous scaffold. The proliferation of fibroblast on the PHBV/SF nanofibrous scaffold with 50/50 was higher than that on the pure PHBV nanofibrous scaffold. Our results indicated that the PHBV/SF nanofibrous scaffold with 50/50 may be a better candidate for biomedical applications such as skin tissue engineering and wound dressing. POLYM. ENG. SCI., 55:907–916, 2015. © 2014 Society of Plastics Engineers