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Preparation and characterization of ampicillin‐incorporated electrospun polyurethane scaffolds for wound healing and infection control
Author(s) -
Sabitha M.,
Rajiv Sheeja
Publication year - 2015
Publication title -
polymer engineering and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.503
H-Index - 111
eISSN - 1548-2634
pISSN - 0032-3888
DOI - 10.1002/pen.23917
Subject(s) - materials science , nanofiber , electrospinning , ampicillin , polyurethane , hacat , wound healing , antibiotics , composite material , polymer , microbiology and biotechnology , chemistry , surgery , medicine , biochemistry , in vitro , biology
Electrospinning is a desired method to produce interconnected flexible nanofibrous structures suitable for tissue engineering, drug delivery, and wound healing. Ampicillin‐loaded polyurethane (PU) nanofiber mats were electrospun with the antibiotic dispersed in well‐oriented nanofibers. The identification of functional groups, molecular interactions and surface morphology of the fibers were analyzed using Infrared, Raman, 1 H NMR, SEM, and TEM. Weak interactions exist between the functional groups of ampicillin and PU in electrospun fibers at ratios 1:10, 1.5:10, and 2:10. The effect of β‐lactam antibiotic ampicillin on the characteristics of electrospun PU was studied using XRD, TGA, and DSC. Their antibacterial property is proved by good zone of inhibition against Staphylococcus aureus and Klebsiella pneumonia . Cytotoxicity tests on the electrospun scaffolds were performed with normal human keratinocyte cells (HaCaT cells). Results indicate that ampicillin‐incorporated PU scaffolds are well suited for applications in wound healing and infection control. POLYM. ENG. SCI., 55:541–548, 2015. © 2014 Society of Plastics Engineers