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A rapid and quantitative mass spectrometry method for determining the concentration of acylcarnitines and aminoacids in amniotic fluid
Author(s) -
Braida Laura,
Crovella Sergio,
Boniotto Michele,
Luchesi Ariella,
de Vonderweid Umberto,
Casetta Bruno,
Amoroso Antonio
Publication year - 2001
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.93
Subject(s) - amniotic fluid , tandem mass spectrometry , mass spectrometry , chromatography , chemistry , quantitative analysis (chemistry) , pregnancy , fetus , biology , genetics
We describe a quantitative, rapid, sensitive and reproducible tandem mass spectrometry (MSMS) method for the one‐step detection of aminoacid (AAs) and acylcarnitine (ACs) concentrations in amniotic fluid. This technology is quicker and more sensitive than other methods used to date since it is possible to determine very low AA and AC concentrations in samples simultaneously in a single run. The high degree of automation allows a large number of pregnancies to be screened for metabolic defects in a very short time. Copyright © 2001 John Wiley & Sons, Ltd.