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Prenatal diagnosis of two rare de novo structural aberrations of the Y chromosome: cytogenetic and molecular analysis
Author(s) -
Velissariou Voula,
Antoniadi Thalia,
Patsalis Philippos,
Christopoulou Stavroula,
Hatzipouliou Athina,
Donoghue Jackie,
Bakou Katerina,
Kaminopetros Petros,
Athanassiou Vassilis,
Petersen Michael B.
Publication year - 2001
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.79
Subject(s) - fluorescence in situ hybridization , biology , azoospermia factor , y chromosome , genetics , chromosome , microbiology and biotechnology , prenatal diagnosis , chromosome 21 , azoospermia , polymerase chain reaction , karyotype , gene , fetus , infertility , pregnancy
Two rare de novo structural aberrations of the Y chromosome were detected during routine prenatal diagnosis: a satellited non‐fluorescent Y chromosome (Yqs), the first de novo Yqs to be reported in a fetus, and a terminal deletion of the Y chromosome long arm del(Y)(q11). In both cases detailed cytogenetic and molecular analyses were undertaken. In the case of the Yqs it was demonstrated by fluorescence in situ hybridization (FISH) that the satellites were derived from chromosome 15. In the case of the del(Yq), it was shown with molecular analysis by polymerase chain reaction (PCR) amplification of sequence‐tagged sites (STS‐PCR) that the deleted portion of the long arm of chromosome Y included the azoospermia factor loci, AZFb and AZFc. The clinical significance of these findings is discussed. Copyright © 2001 John Wiley & Sons, Ltd.