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The influence of needle and syringe size on chorionic villus sampling of term placentae: a randomised trial
Author(s) -
Cochrane Lindsay,
Ainscough Magdalen,
Alfirevic Zarko
Publication year - 2003
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.752
Subject(s) - syringe , medicine , chorionic villi , placenta , intervillous space , post partum , significant difference , chorionic villus sampling , obstetrics , pregnancy , fetus , biology , first trimester , psychiatry , genetics
Objective To determine the effect of needle and syringe size on the amount of tissue obtained at chorionic villus sampling Methods Two needle sizes, 18 and 20 gauge, and two syringe sizes 5 mL and 20 mL, were used to assess samples from term post‐partum placentae. Each of the four combinations was tested by 25 aspirations. The placentae were divided into 100 grid spaces and each grid space was randomly allocated to a needle/syringe combination. The resulting samples were cleaned to separate the chorionic villi (CV), centrifuged and then weighed. Results Significantly more tissue was obtained with an 18‐g needle compared with a smaller 20‐g needle (median weight difference 1.5 mg, 95% CI 0.8–2.3 mg). More tissue was also obtained with the larger 20‐mL syringe though the impact of the syringe size was less than that of the needle size (median difference 0.8 mg, 95% CI 0–1.6). Conclusion A larger syringe and needle size yields a larger quantity of chorionic villi from the post‐partum term placenta. Copyright © 2003 John Wiley & Sons, Ltd.