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Comparison between fluorescence in situ hybridization (FISH) and quantitative‐fluorescent polymerase chain reaction (QF‐PCR) for the detection of aneuploidies in single blastomeres
Author(s) -
Sato Takeshi,
Ikuta Katsuo,
Sherlock Jon,
Adinolfi Matteo,
Suzumori Kaoru
Publication year - 2003
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.660
Subject(s) - sexing , blastomere , embryo , biology , fluorescence in situ hybridization , fish <actinopterygii> , polymerase chain reaction , in situ hybridization , microbiology and biotechnology , chromosome , aneuploidy , genetics , gene , embryogenesis , messenger rna , fishery
Objectives The aim of our investigation was to compare the efficiencies of the fluorescence in situ hybridization (FISH) and the quantitative‐fluorescent PCR (QF‐PCR) methods for the detection of sexing and numerical chromosome disorders in single blastomeres collected from the same preimplantation human embryos. Methods FISH analysis was carried out on 145 blastomeres from the 79 surplus embryos with probes specific for chromosomes 13, 18, 21, X, and Y. QF‐PCR was performed with each one or two of the primers specific for the same chromosomes on 151 blastomeres from the same embryos obtained from patients undergoing IVF treatment. Results Analyses were possible on 135 blastomeres (93%) by FISH and on 117 blastomeres (77%) by QF‐PCR. Of 65 embryos, which could be analyzed by both methods, 20 embryos (31%) were diagnosed as abnormal. Conclusion The present study shows that FISH tests are more accurate than QF‐PCR assays for the detection of numerical chromosome disorders when performed on single blastomeres. Copyright © 2003 John Wiley & Sons, Ltd.