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Tissuepatch is biocompatible and seals iatrogenic membrane defects in a rabbit model
Author(s) -
Engels Alexander C.,
Joyeux Luc,
Van der Merwe Johannes,
Jimenez Julio,
Prapanus Savitree,
Barrett David W.,
Con Che,
Chowdhury Tina T.,
David Anna L.,
Deprest Jan
Publication year - 2018
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.5191
Subject(s) - biocompatibility , fetoscopy , fetus , fetal membrane , medicine , biomedical engineering , membrane , biocompatible material , andrology , surgery , chemistry , pregnancy , biology , biochemistry , placenta , prenatal diagnosis , organic chemistry , genetics
Objective To evaluate novel sealing techniques for their biocompatibility and sealing capacity of iatrogenic fetal membrane defects in a pregnant rabbit model. Method At day 23 of gestation (term = d31), a standardized fetoscopy was performed through a 14G cannula. The resulting fetal membrane defect was closed with condensed collagen, collagen with fibrinogen, Tissuepatch, Duraseal, or a conventional collagen plug (Lyostypt) as reference. At d30, the fetuses were harvested and full thickness fetal membrane samples were analyzed. The study consisted of 2 consecutive parts: (1) biocompatibility testing by fetal survival, apoptosis, and infiltration of polymorphonuclear cells in the membranes and (2) the efficacy to seal fetal membrane defects. Results Three sealants (collagen with fibrinogen, Duraseal, or Lyostypt) were associated with a higher fetal mortality compared to control unmanipulated littermates and hence were excluded from further analysis. Tissuepatch was biocompatible, and amniotic fluid levels were comparable to those of control untouched littermates. Compared to the condensed collagen, Tissuepatch was also easier in surgical handling and induced limited cell proliferation. Conclusion Tissuepatch had the best biocompatibility and efficacy in sealing an iatrogenic fetal membrane defect in the pregnant rabbit compared to other readily available sealants.