Premium
Trophoblast retrieval and isolation from the cervix (TRIC) is unaffected by early gestational age or maternal obesity
Author(s) -
Fritz Rani,
KohanGhadr Hamid Reza,
Sacher Alex,
Bolnick Alan D.,
Kilburn Brian A.,
Bolnick Jay M.,
Diamond Michael P.,
Drewlo Sascha,
Armant D. Randall
Publication year - 2015
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.4681
Subject(s) - trophoblast , pregnancy , obstetrics , gestational age , cervix , preeclampsia , inner cell mass , medicine , body mass index , gynecology , andrology , blastocyst , placenta , biology , fetus , embryo , genetics , cancer , embryogenesis
Objective The objective of this study is to evaluate whether trophoblast yield obtained by trophoblast retrieval and isolation from the cervix (TRIC) is affected by pregnancy outcome, gestational age (GA) at retrieval, maternal body mass index (BMI), parity, or maternal age. Methods TRIC was performed on 224 ongoing pregnancies between 5 and 20 weeks of GA. Trophoblast cells were isolated from cervical cells using anti‐human leukocyte antigen‐G antibody coupled to magnetic nanoparticles. Purity was assessed by the percentage of isolated cells that express β‐hCG. Patient records were monitored until delivery, and pregnancy outcomes were determined. Trophoblast yield was compared with GA at time of collection, maternal BMI, parity, maternal age, and outcome of pregnancy, using linear regression. Results There was no effect of GA, maternal BMI, parity, and maternal age on trophoblast yield. Trophoblast yield decreased significantly with early pregnancy loss compared with uncomplicated pregnancies that delivered at term. Trophoblast yield with preeclampsia or intrauterine growth restriction was decreased compared with healthy term outcomes; however, they did not reach statistical significance. Conclusions If TRIC becomes available as a method for non‐invasive prenatal testing, our data demonstrate that it is unaffected by BMI and is useful as early as 5 weeks of GA. © 2015 John Wiley & Sons, Ltd.