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Single‐tube nonaplex microsatellite PCR panel for preimplantation genetic diagnosis of Hb Bart's hydrops fetalis syndrome
Author(s) -
Chen Min,
Chan Jerry K. Y.,
Nadarajah Sadhana,
Tan Arnold S. C.,
Chan Melinda L. H.,
Mathew Joyce,
Saw Eugene E. L.,
Lim Cheryl,
Wong Wendy,
Cheah Felicia S. H.,
Law HaiYang,
Wong PengCheang,
Chong Samuel S.
Publication year - 2015
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.4568
Subject(s) - hydrops fetalis , microsatellite , preimplantation genetic diagnosis , genetics , biology , genetic linkage , allele , haplotype , genetic marker , microbiology and biotechnology , gene , pregnancy , fetus
Objective To develop a single‐tube multi‐marker assay for improved preimplantation genetic diagnosis (PGD) of deletional and/or non‐deletional Hb Bart's hydrops fetalis syndrome, providing haplotype confirmation of deletional status, and maximization of linkage informativity. Methods We performed in silico mining to identify novel microsatellites within 1 Mb flanking the alpha‐globin gene cluster, and optimized a single‐tube assay combining detection of α 0 ‐thalassemia deletions with multi‐marker linkage analysis. We performed validation on 100 single cells prior to clinical PGD application. Results Of 42 markers encompassing the α‐globin gene cluster that were identified in silico , 9 were highly polymorphic (0.68 ≤ polymorphism information content ≤ 0.92; 0.66 ≤ H o ≤ 0.90; 10 ≤ alleles ≤ 35) and optimized to co‐amplify directly from a single cell. A validation analysis of 100 single lymphoblasts yielded 100% amplification success for all markers, and individual marker allele drop‐out (ADO) rates of 0–5%. Clinical application of the assay in PGD for Hb Bart's (2 cases/cycles) resulted in a twin pregnancy and healthy live birth of two baby girls. Conclusions This single‐tube nonaplex microsatellite PCR panel can be applied directly to PGD of most deletional Hb Bart's without the need for deletion‐specific customization, and to linkage‐based PGD of non‐deletional Hb Bart's. © 2015 John Wiley & Sons, Ltd.