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Effect of labor on plasma concentrations and postpartum clearance of cell‐free, pregnancy‐associated, placenta‐specific microRNAs
Author(s) -
Morisaki Shintaro,
Miura Kiyonori,
Higashijima Ai,
Abe Shuhei,
Miura Shoko,
Hasegawa Yuri,
Yoshida Atsushi,
Kaneuchi Masanori,
Yoshiura Kohichiro,
Masuzaki Hideaki
Publication year - 2015
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.4479
Subject(s) - placenta , pregnancy , microrna , medicine , andrology , obstetrics , fetus , endocrinology , biology , gene , biochemistry , genetics
Objective This study aimed to investigate the effect of labor on plasma concentrations of cell‐free, pregnancy‐associated, placenta‐specific microRNAs (miRNAs) before and after delivery. Method In the non‐labor group (32 women), cesarean section (C/S) was performed before the beginning of labor. In the labor group (32 women), C/S was performed after the beginning of labor. Plasma concentrations of cell‐free, pregnancy‐associated, placenta‐specific miRNAs (miR‐515‐3p, miR‐517a, miR‐517c, and miR‐518b) were measured by real‐time quantitative PCR. Each miRNA concentration was compared between the non‐labor and labor groups. Results Before C/S, plasma concentrations of cell‐free, pregnancy‐associated, placenta‐specific miRNAs in the labor group were significantly higher than those in the non‐labor group ( P = 0.001 for 515‐3p, P = 0.002 for 517a, P = 0.001 for 517c, and P = 0.003 for 518b). Twenty‐four hours after delivery, plasma concentrations of cell‐free, pregnancy‐associated, placenta‐specific miRNAs in the labor group were significantly higher than those in the non‐labor group ( P = 0.002 for 515‐3p, P = 0.017 for 517a, P = 0.043 for 517c, and P = 0.009 for 518b). Conclusion The presence of labor affects cell‐free, pregnancy‐associated, placenta‐specific miRNA levels in maternal plasma. Labor also affects postpartum clearance of these miRNAs 24 h after delivery. © 2014 John Wiley & Sons, Ltd.