Premium
Differences in IGF axis‐related proteins in amniotic fluid of trisomy 21 and trisomy 18 using a multiple reaction monitoring approach
Author(s) -
Lai Guangrui,
Zhang Yuanyuan,
Wang Leitong,
Zhang Bijun,
Zheng Gaoyan,
Wang Xuanyi,
Zhao Xiaoqiu,
Gao Haiming,
Zhao Yanyan
Publication year - 2014
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.4443
Subject(s) - trisomy , fetus , amniotic fluid , aneuploidy , western blot , down syndrome , biology , andrology , endocrinology , medicine , pregnancy , genetics , chromosome , gene
Objective Trisomy 21 and trisomy 18 are the two most common chromosomal anomalies in live births. To find new biomarkers for aneuploidies and pathogenesis of fetal malformations, we measured insulin‐like growth factor (IGF) axis‐related proteins in amniotic fluid (AF) of pregnant women carrying trisomies 21 or 18 affected fetuses using multiple reaction monitoring (MRM) approach. Method Eighty‐five AF samples from pregnant women carrying either trisomy 21, trisomy 18, or normal fetuses were collected. IGF axis‐related proteins in AF after serial treatments were quantitated with MRM method. The differential protein levels were also confirmed by western blot in AF without any treatment. Results The IGF type I receptor and pregnancy‐associated plasma protein‐A in AF of trisomy 21 (1.35 ± 0.32 and 13.36 ± 3.64 µg/mg protein) and trisomy 18 (1.39 ± 0.40 and 12.80 ± 1.84 µg/mg protein) were decreased versus normal controls (2.16 ± 0.59 and 23.77 ± 6.18 µg/mg protein). IGF binding protein 5 was reduced in trisomy 18 (1.47 ± 0.33 vs 2.36 ± 0.77 µg/mg protein). These alterations were confirmed by western blot. The other proteins showed no significant difference between the three groups. Conclusion Our data suggested that MRM can provide a powerful platform for the identification of biomarkers in AF that have crucial developmental effects in the aneuploid fetus. © 2014 John Wiley & Sons, Ltd.