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Predominantly placenta‐expressed mRNAs in maternal plasma as predictive markers for twin–twin transfusion syndrome
Author(s) -
Miura Kiyonori,
Higashijima Ai,
Miura Shoko,
Mishima Hiroyuki,
Yamasaki Kentaro,
Abe Shuhei,
Hasegawa Yuri,
Kaneuchi Masanori,
Yoshida Atsushi,
Kinoshita Akira,
Yoshiura Kohichiro,
Masuzaki Hideaki
Publication year - 2014
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.4307
Subject(s) - human placental lactogen , placenta , glycoprotein , placental lactogen , messenger rna , andrology , hormone , biology , medicine , chorionic villi , endocrinology , pregnancy , microbiology and biotechnology , gene , fetus , biochemistry , genetics
ABSTRACT Objective This study aimed to identify a set of predominantly placental (PP) mRNAs, which are associated with later‐developing twin‐to‐twin transfusion syndrome (TTTS). Method First, out of 50 PP mRNAs we previously reported, we select target mRNAs that are ordinarily detectable in maternal plasma. Plasma concentrations of these PP mRNAs were measured in monochorionic diamniotic twin (MCDA‐T) pregnancies complicated by TTTS later ( n = 11) and in uncomplicated MCDA‐T pregnancies ( n = 17). Finally, the diagnostic values of the PP mRNAs in plasma were evaluated. Results From 50 PP mRNAs, nine [human placental lactogen ( hPL ); pregnancy‐specific glycoproteins 2 ( PSG2 ); human pregnancy‐specific glycoproteins 3 ( PSG3 ); syncytin ; syncytin 2 ; retinoic acid‐induced 14; A disintegrin and metalloproteinase domain‐containing protein 12 ( ADAM12 ); chorionic glycoprotein hormones, alpha polypeptide; and chorionic glycoprotein hormones, and beta polypeptide] were selected as target mRNAs. Changes in six PP mRNAs [increased hPL , PSG2 , and PSG3 and decreased syncytin , syncytin2 , and ADAM12 ] in maternal plasma were detected in MCDA‐T pregnant women who subsequently developed TTTS. Finally, mRNA signatures gave elevated AUCs ( hPL / PSG2 : 0.8717; hPL / PSG3 : 0.8449; hPL / ADAM12 : 0.8396) compared with single hPL mRNA. Conclusion Quantitative aberration of plural cell‐free PP mRNAs in maternal plasma precedes the appearance of clinically apparent TTTS. This suggests that pathophysiological changes in the placenta are associated with morbid conditions of TTTS. © 2013 John Wiley & Sons, Ltd.