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Identification of circulating fetal cell markers by microarray analysis
Author(s) -
Brinch Marie,
Hatt Lotte,
Singh Ripudaman,
Møller Kristine,
Sommer Steffen,
Uldbjerg Niels,
Christensen Britta,
Kølvraa Steen
Publication year - 2012
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.3894
Subject(s) - fetus , laser capture microdissection , biology , cell sorting , fluorescence in situ hybridization , stem cell marker , cell , microdissection , andrology , blood cell , nucleated red blood cell , microbiology and biotechnology , microarray , prenatal diagnosis , red blood cell , pathology , immunology , flow cytometry , gene , gene expression , pregnancy , medicine , genetics , chromosome
Objective Different fetal cell types have been found in the maternal blood during pregnancy in the past, but fetal cells are scarce, and the proportions of the different cell types are unclear. The objective of the present study was to identify specific fetal cell markers from fetal cells found in the maternal blood circulation at the end of the first trimester. Method Twenty‐three fetal cells were isolated from maternal blood by removing the red blood cells by lysis or combining this with removal of large proportions of maternal white blood cells by magnetic‐activated cell sorting. Fetal cells identified by XY fluorescence in situ hybridization and confirmed by reverse‐color fluorescence in situ hybridization were shot off microscope slides by laser capture microdissection. The expression pattern of a subset of expressed genes was compared between fetal cells and maternal blood cells using stem cell microarray analysis. Results Twenty‐eight genes were identified as fetal cell marker candidates. Conclusion Of the 28 fetal marker candidate genes, five coded for proteins, which are located on the outer surface of the cell membrane and not expressed in blood. The protein product of these five genes, MMP14, MCAM, KCNQ4, CLDN6, and F3, may be used as markers for fetal cell enrichment. © 2012 John Wiley & Sons, Ltd.