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A longer tracheal occlusion period results in increased lung growth in the nitrofen rat model
Author(s) -
Beck Veronika,
Davey Marcus G.,
Mayer Steffi,
Froyen Guy,
Deckx Sebastiaan,
Klaritsch Philipp,
Roubliova Xenia I.,
Petersen Scott G.,
Deprest Jan A.
Publication year - 2012
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.2881
Subject(s) - congenital diaphragmatic hernia , nitrofen , lung , medicine , fetus , diaphragmatic breathing , diaphragmatic hernia , occlusion , hernia , cardiology , pathology , anatomy , surgery , pregnancy , biology , genetics , alternative medicine
Objective Prenatal tracheal occlusion (TO) promotes lung growth and is applied clinically in fetuses with severe congenital diaphragmatic hernia. Limited data are available regarding the effect of duration of TO on lung development. Our objective was to evaluate the effects of long (2 and 2.5 days) versus short (1 day) TO on lung development in rats with nitrofen‐induced diaphragmatic hernia. Method Nitrofen was administered on embryonic day (ED) 9 and fetal TO performed either on ED18.5, 19 or 20 (term = 22 days). Sham‐operated and untouched littermates served as controls. On ED21, lungs were harvested and only fetuses with a left‐sided diaphragmatic defect were included in further analyses. Results Lung–body‐weight ratio incrementally increased with the duration of TO. Increased proliferation following long TO was confirmed by immunohistochemistry and qRT‐PCR for the proliferation marker Ki‐67. Irrespective of duration, TO induced more complex airway architecture. Medial wall thickness of pulmonary arteries was thinner after long rather than short TO. Conclusion In the nitrofen rat model of congenital diaphragmatic hernia, a longer period of TO leads to enhanced lung growth and less muscularized pulmonary arteries. © 2011 John Wiley & Sons, Ltd.