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Circulating cell‐free DNA levels increase variably following chorionic villus sampling
Author(s) -
Vora Neeta L.,
Johnson Kirby L.,
Peter Inga,
Tighiouart Hocine,
Ralston Steven J.,
Craigo Sabrina D.,
Bianchi Diana W.
Publication year - 2010
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.2456
Subject(s) - chorionic villus sampling , cell free fetal dna , chorionic villi , andrology , medicine , pregnancy , prenatal diagnosis , obstetrics , fetus , biology , genetics
Objective Cell‐free fetal DNA (cffDNA) in maternal plasma results from degradation of fetal and/or placental cells. Our objective was to determine if chorionic villus sampling (CVS) causes increased release of fetal and/or maternal DNA. Methods Fifty‐two pregnant women were recruited prior to CVS, performed for clinical indications, at 10 5/7 to 13 2/7 weeks. Maternal blood was collected before and within 15 min after CVS. cffDNA was extracted from plasma. Real‐time polymerase chain reaction (PCR) amplification of glyceraldehyde‐3‐phosphate dehydrogenase ( GAPDH ) and the Y chromosome sequence DYS1 were used as measures of total and fetal DNA, respectively. All samples were analyzed in triplicate without knowledge of fetal gender. Results Sensitivity of DYS1 detection in male fetuses was 100% ( n = 30); specificity in female fetuses was 100% ( n = 22). While a majority of women had > 50% post‐procedure increases in both fetal and total DNA, some showed post‐procedure decreases. However, overall median proportional increases were not statistically significant. Gestational age (GA), placental location, and individual CVS operator did not correlate with changes in DNA levels. Conclusions While there were no statistically significant overall changes in DNA levels after CVS, as‐yet undiscovered variables may influence the extent of post‐procedure release of cell‐free DNA in the circulation of pregnant women. Copyright © 2010 John Wiley & Sons, Ltd.