Combined QF‐PCR and MLPA molecular analysis of miscarriage products: an efficient and robust alternative to karyotype analysis

Objectives To replace G‐banded chromosome analysis for miscarriage products with a combined molecular approach: QF‐PCR and MLPA, to increase efficiency, reduce costs, and improve the diagnostic success rate for these samples. Methods A review of 10 years of karyotype results for miscarriages products indicated that 2.7% of nonmosaic chromosome imbalance would not be detected by the molecular approach. The molecular approach was validated on 117 samples in parallel with karyotype analysis; no discrepancies were detected. The molecular approach was implemented in September 2007, and in the first 18 months 500 samples were processed. Results In 500 samples, 117 samples (23%) were abnormal. Of these abnormalities, 64% were trisomies, 12% triploid, 11% monosomy X and 13% other abnormalities. When compared to karyotype analysis, the success rate was higher (95% cf 70%) and the reporting time was lower (88% within 28 days cf 79%). In addition, efficiency was higher as labour‐intensive cell culture and karyotyping were replaced by batch testing and automated analysis. Conclusions This molecular approach is less labour‐intensive, allows a higher sample throughput and has a higher success rate than karyotype analysis; it is therefore an efficient and cost‐effective diagnostic testing strategy for miscarriage products. Copyright © 2009 John Wiley & Sons, Ltd.