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In vitro evaluation of the ability of platelet‐rich plasma to seal an iatrogenic fetal membrane defect
Author(s) -
Lewi L.,
Liekens D.,
Heyns L.,
Poliard E.,
Beutels E.,
Deprest J.,
Hoylaerts M. F.
Publication year - 2009
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.2249
Subject(s) - amnion , fetal membrane , membrane , platelet rich plasma , in vitro , fetus , fetoscopy , cell , explant culture , microbiology and biotechnology , andrology , amniotic fluid , cell growth , platelet , biology , medicine , immunology , placenta , prenatal diagnosis , pregnancy , biochemistry , genetics
Objectives The purpose of the study is to evaluate the ability of platelet‐rich plasma (PRP) to seal an iatrogenic fetal membrane defect. Methods First, we evaluated the stability of a PRP plug in an amniotic fluid environment. Further, we evaluated the sealing capability of PRP plugs in an in vitro model that mimics a fetoscopic membrane defect. Finally, we examined its influence on membrane repair and cell proliferation in monolayer cell cultures and amnion‐chorion tissue explants. Results PRP plugs persisted in an amniotic fluid for a median time of 7 weeks. PRP plugs also provided waterproof sealing of a fetoscopic membrane defect. Finally, PRP stimulated cell proliferation in a monolayer cell culture and provided a good matrix for cell proliferation and migration in amnion–chorion tissue explants. Conclusion Our in vitro experiments suggest that PRP plugs may provide a long‐lasting, waterproof sealing of fetal membrane defects and stimulate fetal membrane repair. Copyright © 2009 John Wiley & Sons, Ltd.