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The effect of maternal betamethasone and fetal tracheal occlusion on pulmonary vascular morphometry in fetal rabbits with surgically induced diaphragmatic hernia: a placebo controlled morphologic study
Author(s) -
Roubliova Xenia I.,
Lewi Paul J.,
Verbeken Eric K.,
Vaast Pascal,
Jani Jacques C.,
Lu Huiqi,
Tibboel Dick,
Deprest Jan A.
Publication year - 2009
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.2243
Subject(s) - congenital diaphragmatic hernia , medicine , fetus , betamethasone , diaphragmatic hernia , fetal surgery , lung , pulmonary sequestration , surgery , anesthesia , hernia , in utero , pregnancy , genetics , biology
Objectives We studied the vascular effects of betamethasone (BM) and/or tracheal occlusion (TO) in fetal rabbits with surgically induced congenital diaphragmatic hernia (CDH). Methods At day 23 (pseudoglandular phase; term = 31 d), 54 ovarian‐end fetuses from 27 does underwent induction of CDH. Thirteen did receive either 0.05 mg/kg BM, on days 28 and 29 with a 24‐h interval, or 14 saline [controls (CTR)]. At day 28, one ovarian‐end fetus underwent TO and harvesting was at term. In total, we compared (ANOVA) lung‐to‐body weight ratio (LBWR) and vascular morphometric indices in survivors from the following groups ( n ‐ number alive at delivery): CDH (9); CDH + TO (10); unoperated controls (14); CDH + BM (10); CDH + TO (9); controls CTR + BM (13). Results Maternal BM had no effect on LBWR. LBWR was comparable to normal in CDH fetuses undergoing TO. Both TO and BM have an effect on medial thickening due to CDH which is larger when both interventions are combined. Conclusions Both TO and BM lessen peripheric muscularization present in CDH lungs and their effect is cumulative. Copyright © 2009 John Wiley & Sons, Ltd.