Premium
The presence of trophoblastic cells in intrauterine lavage samples: lack of correlation with maternal and obstetric characteristics
Author(s) -
Cioni Riccardo,
Bussani Cecilia,
Conti Elisa,
Buzzoni Carlotta,
Bucciantini Sandra,
Mattei Alberto,
Scarselli Gianfranco
Publication year - 2008
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.2131
Subject(s) - medicine , andrology , obstetrics , pregnancy , biology , genetics
Abstract Objectives To investigate the correlation between maternal, obstetric and sample characteristics and the quality (i.e. yield of trophoblastic cells) of intrauterine lavage (IUL) samples. Methods We collected 202 IUL samples from women scheduled for first trimester termination of pregnancy (TOP). Trophoblastic cells were isolated from IUL samples and used for DNA analysis by a multiplex quantitative fluorescent polymerase chain reaction (QF‐PCR) assay. A multivariate logistic regression analysis was performed, and a p < 0.05 was considered statistically significant. Results The presence of trophoblastic cells in IUL samples was documented in 151/202 cases (74.7%). Blood contamination of IULs was the only characteristic to positively correlate with the presence of trophoblasts ( p = 0.039; OR: 1.99; 95% CI: 1.03–3.82). Conclusions The correlation between the presence of contaminating blood and trophoblastic cells would indirectly confirm the hypothesis that IUL might act as a mini‐CVS. The high yield rate of trophoblasts irrespective of maternal characteristics and past obstetric history would support the clinical use of this sampling technique, provided that its safety is clearly defined. Copyright © 2008 John Wiley & Sons, Ltd.