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Gene expression in chorionic villous samples at 11 weeks' gestation from women destined to develop preeclampsia
Author(s) -
Farina Antonio,
Sekizawa Akihiko,
De Sanctis Paola,
Purwosunu Yuditiya,
Okai Takashi,
Cha Dong Hyun,
Kang Jin Hee,
Vicenzi Claudia,
Tempesta Annalisa,
Wibowo Noroyono,
Valvassori Luisella,
Rizzo Nicola
Publication year - 2008
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.2109
Subject(s) - preeclampsia , endoglin , placental growth factor , vascular endothelial growth factor , angiogenesis , andrology , pathogenesis , placenta , gestation , biology , enos , pregnancy , medicine , endocrinology , intrauterine growth restriction , fetus , genetics , cd34 , nitric oxide , nitric oxide synthase , vegf receptors , stem cell
Abstract Objective To evaluate the direct alterations in mRNA expression among chorionic villous samples from 11 weeks' pregnant women who would develop preeclampsia (PE) later in the pregnancy. Method Case‐control study encompassing five women destined to develop PE [cases matched 1:5 for gestational age (GA) with 25 controls]. We quantified mRNA expression on tissue samples from chorionic villous sampling (CVS) of normal and PE patients. We then assessed mRNA expressions of vascular endothelial growth factor (VEGFA), VEGFA receptor 1 (Flt‐1), endoglin (Eng), placental growth factor (PlGF), transforming growth factor‐β1 (TGF‐β1), heme oxygenase‐1 (HO‐1) and superoxide dismutase (SOD). Data were analyzed by nonparametric rank analysis. Results For all the mRNA species considered in this study, all the mean observed ranks in the PE group were significantly altered compared to the rank expectation among controls. mRNA for Eng and TGF‐β1 were the markers with the highest degree of aberration in PE, in respect to controls. The results are consistent with those already reported for the corresponding circulating proteins. mRNA for HO‐1 and SOD were instead associated with the lowest aberration. Conclusion It is assumed that the pathogenesis of PE is associated with pathophysiological alterations to trophoblasts in early gestation. Our study has directly proved that gene expressions relating to angiogenesis or oxidative stress are altered in the first trimester trophoblasts that go on to develop PE later. These results would put the basis for a possible screening method for PE by using residual CVS. Copyright © 2008 John Wiley & Sons, Ltd.