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Enrichment of collagen plugs with platelets and amniotic fluid cells increases cell proliferation in sealed iatrogenic membrane defects in the foetal rabbit model
Author(s) -
Liekens Daisy,
Lewi Liesbeth,
Jani Jacques,
Heyns Liesbeth,
Poliard Eline,
Verbist Godelieve,
OchsenbeinKölble Nicole,
Hoylaerts Marc,
Deprest Jan
Publication year - 2008
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.2010
Subject(s) - amniotic fluid , heterologous , andrology , amniotic stem cells , fetal membrane , chemistry , cell , platelet , fetus , microbiology and biotechnology , biology , immunology , placenta , medicine , in vitro , endothelial stem cell , pregnancy , biochemistry , genetics , adult stem cell , gene
Objectives The purpose of this study was to evaluate cell proliferation in platelet‐enriched collagen plugs with and without addition of amniotic fluid‐derived heterologous foetal cells to seal an iatrogenic membrane defect in the foetal rabbit model. Methods Amniotic fluid cells were harvested from three donor does at 23 days of gestation (term = 32 days) and labelled with carboxyfluorescein diacetate succinimidyl ester (CFDA‐SE). In 42 other does, foetal membrane defects were induced by foetoscopic needle puncture at 23 days of gestation, and closed with either a platelet‐enriched collagen plug with ( n = 44) or without ( n = 32) amniotic fluid cells. At 30 days of gestation, the defects were harvested and assessed microscopically. Results The plugs enriched with heterologous amniotic fluid cells more commonly had proliferating cells in the centre of the plug than those without cell addition. CFDA‐SE labelling confirmed the presence of heterologous amniotic fluid cells over the entire membrane plug. Cell typing showed a mixture of fibroblasts and epithelial cells at the wound edges, whereas in the centre, there was an abundance of fibroblasts. Conclusion When sealing iatrogenic membrane defects in the foetal rabbit model, enrichment of collagen plugs with platelets and amniotic fluid‐derived heterologous foetal cells increases local cell proliferation. Copyright © 2008 John Wiley & Sons, Ltd.