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Chromosome 18 analysis by fluorescence in situ hybridization (fish) in human blastomeres of abnormal embryos after in vitro fertilization (ivf) attempt
Author(s) -
Bergere M.,
Selva J.,
Baud M.,
Volante M.,
Martin B.,
Hugues J. N.,
Olivennes F.,
Frydman R.,
Auroux M.
Publication year - 1995
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970150908
Subject(s) - pronucleus , embryo , fluorescence in situ hybridization , biology , blastomere , chromosome , ploidy , genetics , andrology , aneuploidy , in vitro fertilisation , human fertilization , in situ hybridization , chromosome abnormality , microbiology and biotechnology , zygote , karyotype , embryogenesis , medicine , gene , gene expression
Abstract We performed fluorescence in situ hybridization (FISH) with a chromosome 18‐specific probe on human abnormal cleaved embryos, fertilized either by two spermatozoa and exhibiting three pronuclei (3 PN) or normally fertilized and exhibiting two pronuclei (2 PN) with subsequent severe fragmentation and/or blocking. The aim of the study was to evaluate the incidence of chromosome 18 anomalies among these embryos, in order to evaluate the FISH efficiency on such material and to obtain more precise and complete data than those obtained with classical cytogenetic analysis. For the 3 PN cleaved embryos, FISH confirmed the frequent regulation towards diploidy (25 per cent) and the high frequency of mosaics (53 per cent). For the 2 PN blocked or damaged embryos, FISH permitted chromosome evaluation, which was otherwise impossible with classical cytogenetic techniques: we also found a high mosaic frequency (45 per cent) with these embryos. If this frequency were the same for normally developing embryos, it would be a major obstacle to the reliability of either chromosomal or genetic preimplantation diagnosis.