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Molecular evidence of fetal‐derived chromosome 21 markers (STRs) in transcervical samples
Author(s) -
Adinolfi Matteo,
Sherlock Jon,
Soothill Peter,
Rodeck Charles
Publication year - 1995
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970150108
Subject(s) - fetus , polymerase chain reaction , fluorescence in situ hybridization , biology , prenatal diagnosis , microbiology and biotechnology , in situ hybridization , hybridization probe , andrology , gestation , chromosome , pathology , dna , medicine , pregnancy , genetics , gene , messenger rna
Transcervical cells (TCCs), collected by flushing or aspiration at 8–13 weeks of gestation, were analysed for the presence of fetal‐derived DNA sequences. DNA extracted from maternal peripheral blood, TCC samples, and placental tissue was amplified by the polymerase chain reaction (PCR) to detect small tandem repeat (STR) markers specific to chromosome 21. STR products of fetal origin could be clearly observed in four TCC samples. TCC samples collected by flushing or aspiration were also analysed by fluorescent in situ hybridization (FISH) using X and Y probes simultaneously: 46,XY cells could be detected in all TCC samples obtained from mothers with male fetuses.