Premium
Identification of abnormal chromosomal complement in formalin‐fixed, paraffin‐embedded placental tissue
Author(s) -
Cowles Tracy A.,
Elder Frederick F. B.,
Taylor Suzanne
Publication year - 1995
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970150106
Subject(s) - karyotype , biology , fluorescence in situ hybridization , chromosome , fish <actinopterygii> , in situ hybridization , hybridization probe , pathology , aneuploidy , dna , genetics , medicine , gene , gene expression , fishery
The objective of this project was to assess the efficacy of fluorescence in situ hybridization (FISH) with chromosome‐specific DNA probes to identify chromosome number in formalin‐fixed, paraffin‐embedded placental specimens. Using this approach, 75 per cent of the karyotypes in 20 formalin‐fixed placental samples (comprising aneuploids, triploids, and normals) were correctly identified. As this technology improves, the ability to obtain information regarding chromosomal abnormalities in formalin‐fixed, paraffin‐embedded placental tissue should improve as well. This technology can potentially provide important cytogenetic information even when fresh tissue is not available for standard karyotypic analysis.