Premium
Flow cytometric method to measure urea‐resistant alkaline phosphatase from blood neutrophils for use in the prenatal screening of Down's syndrome
Author(s) -
Agrawal Yash P.,
Ylikangas Päivi,
Parviainen Markku T.,
Penttilä Ilkka M.
Publication year - 1994
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970141207
Subject(s) - alkaline phosphatase , flow cytometry , prenatal diagnosis , urea , medicine , down syndrome , andrology , immunology , pregnancy , obstetrics , fetus , endocrinology , chemistry , biology , biochemistry , enzyme , genetics , psychiatry
Abstract The histochemical measurement of urea‐resistant alkaline phosphatase from maternal blood neutrophils is known to have a high detection rate for the prenatal detection of Down's syndrome pregnancies. However, because the histochemical method is laborious and subjective to use, it has not gained widespread acceptance in prenatal screening programmes. We present a simple and objective method for the measurement of urea‐resistant alkaline phosphatase by flow cytometry. The method should allow the design of larger studies aimed at evaluating the role of neutrophil urea‐resistant alkaline phosphatase in the prenatal screening for Down's syndrome.