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Applicability of DNA isolated from syncytiotrophoblast vesicles to gene amplification and molecular analysis
Author(s) -
Colucci G.,
Pesenti E.,
Molteni E.,
Lobbiani A.,
De Andreis C.,
Pariani S.,
Rossella F.,
Semprini A. E.,
Simoni G.
Publication year - 1993
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970130504
Subject(s) - syncytiotrophoblast , biology , dna , microbiology and biotechnology , gene , polymerase chain reaction , multiple displacement amplification , exogenous dna , gene duplication , genetics , locus (genetics) , fetus , dna extraction , placenta , pregnancy
Maternal contamination of fetal DNA represents a major problem when highly sensitive molecular techniques are used in the prenatal diagnosis of genetic diseases. For this reason, we have studied the possibility of using DNA isolated from syncytiotrophoblast vesicles as a target of gene amplification (PCR). Three PCR systems were selected which included a repetitive 149 bp fragment of the Y chromosome, the VNTR locus D1S80, and a portion of the β‐globin gene. The results of these experiments indicate that DNA isolated from syncytiotrophoblast vesicles is free of maternal contamination and is suitable for gene amplification and DNA analysis.