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Rapid detection of aneuploidy in uncultured chorionic villus cells using fluorescence in situ hybridization
Author(s) -
Rao P. Nagesh,
Hayworth Rosa,
Cox Kelly,
Grass Frank,
Pettenati Mark J.
Publication year - 1993
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970130402
Subject(s) - aneuploidy , fluorescence in situ hybridization , chorionic villi , in situ hybridization , biology , interphase , in situ , chorionic villus sampling , hybridization probe , karyotype , trisomy , cytogenetics , microbiology and biotechnology , chromosome , fish <actinopterygii> , prenatal diagnosis , genetics , dna , fetus , gene , pregnancy , chemistry , gene expression , organic chemistry , fishery
Rapid detection of aneuploidy using chromosome‐specific repetitive DNA probes and the potential diagnostic accuracy of fluorescence in situ hybridization (FISH) on interphase cells of chorionic villus samples (CVS) are presented. Analyses demonstrated the ability to correctly identify aneuploidy using FISH in uncultured CVS. Our preliminary investigation suggests that this technique offers a significant clinical potential to circumvent problems of culture, time, and cost in cytogenetic analysis.