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Heteroduplex formation: A potential source of genotyping error from PCR products
Author(s) -
Hatcher Sandra L. S.,
Lambert Que Trang,
Teplitz Raymond L.,
Carlson James R.
Publication year - 1993
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970130304
Subject(s) - heteroduplex , genotyping , polymerase chain reaction , computational biology , genetics , biology , genotype , dna , gene
The formation of heteroduplexes from polymerase chain reaction (PCR) products has recently become a diagnostic tool that is routinely used for the prenatal detection of small deletions or insertions in a number of disease‐causing alleles. We present evidence illustrating that heterozygous PCR products can manifest ‘invisible’ heteroduplexes that can ultimately lead to genotyping errors. Justifications for these ‘invisible’ heteroduplexes and requisite parameters to optimize their detection are presented.