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Discriminant analysis for assessing the value of amniotic fluid microvillar enzymes in the prenatal diagnosis of cystic fibrosis
Author(s) -
Szabó Mária,
Münnich Ákos,
Teichmann Farkas,
Huszka Mariann,
Veress Lajos,
Papp Zoltán
Publication year - 1990
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970101202
Subject(s) - cystic fibrosis , amniotic fluid , prenatal diagnosis , alkaline phosphatase , trehalase , medicine , enzyme , pathology , gastroenterology , fetus , pregnancy , obstetrics , biology , biochemistry , genetics
We have analysed the sensitivity, specificity, and reliability of biochemical diagnosis based on microvillar membrane enzyme assay and using discriminant analysis in amniotic fluid samples obtained from 54 pregnancies at high risk for cystic fibrosis and 125 normal pregnancies. Our results show that amniotic fluid trehalase, alkaline phosphatase, alkaline phosphatase isoenzymes and gamma‐glutamyltransferase enzyme activities measured during 16–20 gestational weeks, in spite of their non‐specificity for cystic fibrosis, have a very good predictive value for fetal cystic fibrosis or exclude the possibility of the disease. Overall enzyme activity analysis provided over 90 per cent reliability of the method.