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Prenatal diagnosis of β‐thalassaemia in Mediterranean populations by dot blot analysis with DNA amplification and allele specific oligonucleotide probes
Author(s) -
Ristaldi M. S.,
Pirastu M.,
Rosatelli C.,
Monni G.,
Erlich H.,
Saiki R.,
Cao A.
Publication year - 1989
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970090906
Subject(s) - allele , oligonucleotide , genetics , southern blot , prenatal diagnosis , microbiology and biotechnology , dna , biology , dot blot , oligomer restriction , pregnancy , gene , fetus
In this study, we describe a simple strategy to detect β‐thalassaemia mutations in prospective parents and to make prenatal diagnosis in pregnancies at risk in the Mediterranean population. Screening of prospective parents is carried out by dot blot analysis on enzymatically amplified DNA with a set of oligonucleotide probes complementary to the most common mutations in this population. Prenatal diagnosis is accomplished by the same procedure on enzymatically amplified amniocyte or trophoblast DNA. The main advantages of this procedure are the simplicity, sensitivity (0.05 μg of DNA), and rapidity (12–24 h). Further simplification is obtained by amplification of the DNA from crude amniotic cell lysate. The very low amount of fetal material necessary for this analysis eliminates the need to culture amniotic fluid cells and may decrease the fetal loss rate associated with trophoblast sampling. The number of specific DNA sequences obtained by the amplification procedure allowed us to use non‐radioactive labelled oligonucleotide probes, which have several advantages compared to radioactive probes.