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An improved method of direct chromosome preparation from chorionic villus and high resolution banding technique
Author(s) -
Xiao Sheng,
Liu QuanZhang,
Wang RuBin,
Zhang MaoYing,
Li Pu
Publication year - 1989
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970090808
Subject(s) - colcemid , chromosome , microbiology and biotechnology , biology , chemistry , genetics , cell , gene
The trophoblast was dissociated from the underlying mesenchymal layer either with acetic acid after short‐term prefixation or with mechanical power after fixation twice. The colcemid treatment time was shorted to 16 min and trypsin solution of low pH (6.2) was used for banding. By these steps, the quality of chromosome banding was greatly improved and complete standard chromosome diagnoses were made in 24 of 24 cases. With the modified technique, high resolution banding chromosomes were consistently obtained after short‐term incubation.