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Direct preparations from chorionic villi–relationship between villous morphology and mitotic index
Author(s) -
Romagnano A.,
Featherstone T.,
Sun L.,
Crane J. P.,
Cheung S. W.
Publication year - 1989
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970090603
Subject(s) - mitotic index , chorionic villi , morphology (biology) , mitosis , biology , chromosome , andrology , fetus , pregnancy , genetics , placenta , medicine , gene
It has been postulated that chorionic villi with abundant sprouts have a higher mitotic index and are therefore preferable for obtaining direct chromosome preparations from chorionic villus samples. This theory was tested by correlating villous morphology with mitotic index. Surprisingly, no statistically significant relationship was found. Choice of culture medium, however, was found to be important, with serum‐free RPMI yielding a higher mitotic index than 40 per cent FCS in MEM. We conclude that villous morphology, as assessed in this study, is not a major factor in determining the success of direct chromosome preparations.