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Synchronization of amniotic fluid cells for high resolution cytogenetics
Author(s) -
Qu JY.,
Dallaire L.,
Lemieux N.,
Drouin R.,
Richer C. L.
Publication year - 1989
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970090107
Subject(s) - colcemid , mitotic index , mitosis , bromodeoxyuridine , prometaphase , amniotic fluid , thymidine , biology , cell synchronization , cytogenetics , microbiology and biotechnology , karyotype , andrology , chromosome , metaphase , genetics , cell cycle , medicine , fetus , dna , cell , cell growth , pregnancy , gene
A high resolution technique was applied to amniotic fluid cells by synchronization. After inoculation, the cells were incubated for 30 h in the presence of either thymidine or 5‐bromodeoxyuridine (BrdU). After removal of the blocking agent and addition of a low concentration of thymidine, the cells were incubated for another 6 1/2–7 h, then harvested in prometaphase without colcemid. This technique gives a mitotic index of 3·7 per cent after thymidine synchronization and of 3·2 per cent after BrdU synchronization, and more than half of the mitoses were in the earlier phases with the chromosomes showing more than 550 bands per haploid set. GBG, GTG, and RHG prometaphases are presented. Precise high‐resolution banding of chromosomes of amniotic fluid cells can increase diagnostic accuracy.