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A routine method for storing lymphocytes for repeated isolation of DNA
Author(s) -
Jørgensen Bjarne R.,
Therkelsen A. J.
Publication year - 1988
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970080910
Subject(s) - isolation (microbiology) , dna , computational biology , medicine , biology , computer science , genetics , bioinformatics
A protocol for easy storage and later expansion of lymphocyte populations is given. Compared with methods using transformed cell lines, the method has a number of advantages for repeated production of cells for the isolation of DNA in amounts sufficient for use in diagnostic DNA technology.