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Circulating ‘trophoblast’ cells in pregnancy have maternal genetic markers
Author(s) -
Bertero M. T.,
Camaschella C.,
Serra A.,
Bergui L.,
CaligarisCappio F.
Publication year - 1988
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970080806
Subject(s) - syncytiotrophoblast , prenatal diagnosis , trophoblast , peripheral blood , fetus , antigen , medicine , pregnancy , monoclonal antibody , monoclonal , immunology , antibody , obstetrics , biology , placenta , genetics
A syncytiotrophoblast‐associated antigen identified by the monoclonal antibody (McAb) H315 is detectable on the surface of a low proportion of peripheral blood cells in pregnant women, raising the possibility of a new approach to prenatal diagnosis of genetic disorders. We aimed at verifying the trophoblastic origin of H315 + cells and their use for prenatal diagnosis of β‐thalassaemia. H315 + cells were separated from the peripheral blood of pregnant women: the DNA obtained from these cells in two selected cases was shown to have genetic markers indistinguishable from those of the mother and definitely different from the fetus. Our results suggest that H315 antigen is expressed by maternal cells and that prenatal diagnosis on peripheral blood of the mother using H315 McAb is not feasible.