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Prenatal diagnosis with biotinylated chromosome specific probes
Author(s) -
Guyot Bernard,
Bazin Anne,
Sole Yvette,
Julien Christophe,
Daffos Fernand,
Forestier François
Publication year - 1988
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970080703
Subject(s) - biotinylation , prenatal diagnosis , chromosome , chromosome analysis , obstetrics , medicine , genetics , karyotype , computational biology , biology , fetus , pregnancy , microbiology and biotechnology , gene
We have used a Y‐chromosome specific DNA probe in a controlled study to determine the presence of Y‐chromosome material and to detect numerical abnormalities in uncultured amniotic fluid cells by fluorescent hybridization. Using this non‐radioactive method, we correctly predicted fetal sex within 48 h in all but 3 of 54 cases and identified an XYY syndrome. The technique was previously tested with no false‐positive or false‐negative results on cultured interphase or metaphase nuclei of fetal fibroblasts and adult T‐lymphocytes. Fluorescent in situ hybridization was applied to long‐term fixed cytogenetic preparations up to 44 months old and was shown to be reliable.