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Rapid karyotyping for prenatal diagnosis in the second and third trimesters of pregnancy
Author(s) -
Basaran Seher,
Miny Peter,
Pawlowitzki IvarHarry,
Horst Jürgen,
Holzgreve Wolfgang
Publication year - 1988
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970080413
Subject(s) - prenatal diagnosis , chorionic villi , obstetrics , chorionic villus sampling , fetus , pregnancy , karyotype , medicine , amniotic fluid , third trimester , gynecology , biopsy , aneuploidy , sampling (signal processing) , chromosome , pathology , biology , genetics , filter (signal processing) , gene , computer science , computer vision
Direct chromosome preparations were performed on placental villi obtained by ultrasoundguided needle aspiration between 18 and 37 weeks of pregnancy in 53 patients. The sampling yielded a sufficient amount of tissue with a maximum of two, and in most cases one, insertions. Placental biopsy is easily performed in cases of severe oligohydrammnios, where fetal blood sampling is usually more difficult. Direct karyotyping of placental villi is faster than chromosome analysis from fetal blood or application of the pipette method on amniotic fluid cells, and currently represents the most rapid approach to prenatal diagnosis of chromosomal abnormalities from the first to the third trimester of pregnancy.