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Prenatal diagnosis of β thalassaemia by reverse phase HPLC
Author(s) -
RouyerFessard Ph.,
Beuzard Y.,
Vidaud M.,
John P.,
Mibashan R. S.
Publication year - 1987
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970070304
Subject(s) - high performance liquid chromatography , prenatal diagnosis , chromatography , fetus , globin , hemoglobinopathy , reversed phase chromatography , column chromatography , chemistry , medicine , pregnancy , biology , hemolytic anemia , biochemistry , hemoglobin , genetics
Reverse phase HPLC of radioactive globin chains has been compared to classical carboxy methyl cellulose chromatography for the prenatal diagnosis of β thalassaemia. The two methods correlated highly ( r = 0.97 p < 0.0005) and provided an identical diagnosis for 40 fetal blood samples of fetuses homozygous or heterozygous for β thalassaemia. The HPLC procedure was much faster and required fewer biochemical steps (no globin preparation). It was at least as accurate and more sensitive than the classical chromatography. A single column can be used for 150 analyses and is always ready to be used. Last but not least it is much less expensive than CMC chromatography.