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The effect of oxygen tension on colony formation and cell proliferation of amniotic fluid cells in vitro
Author(s) -
Held Karsten R.,
Sönnichsen Sönke
Publication year - 1984
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970040303
Subject(s) - oxygen tension , laboratory flask , plating efficiency , oxygen , amniotic fluid , andrology , incubation , cell growth , cell culture , membrane , cell , limiting oxygen concentration , chemistry , biology , biochemistry , fetus , medicine , pregnancy , genetics , organic chemistry
The effects of reduced oxygen concentrations in the gas phase over the culture medium on colony formation and cell proliferation were investigated in high and low cell density primary and secondary cultures of amniotic fluid cells. Using two standard culture methods (25 cm 2 plastic flasks and Leighton type tubes) a significantly reduced culture time was observed at high cell density for mass cultures by incubation within a low oxygen tension gas phase (2.5 per cent to 7.5 per cent O 2 ) instead of conventional air (18 per cent O 2 ). At low cell density colony formation was significantly enhanced in cultures grown at reduced oxygen tension. Using gas permeable membranes as support, lowering the oxygen tension from 7.5 per cent to 2.5 per cent yielded an increase in plating efficiency of cells from approximately 5 per cent to 25 per cent, whereas plating efficiency was less than 2 per cent for cells grown at ambient 18 per cent O 2 . It is suggested that low oxygen tension in the gas phase is an effective means of enhancing clonal growth in amniotic fluid cell cultures, thereby reducing both culture time and risk of culture failure.